All current service contracts will be honored. 10. endobj BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559. BD Accuri C6 Plus software features an intuitive user interface. For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. 新一代的Accuri使流式細胞術更加指日可待。 Taiwan North America. Select the BD Accuri CFlow file (.c6) that contains the data you want to view with FCS Express. Please complete the following information to request a demonstration of the BD Accuri™ C6 flow cytometer. User Tracking Installer Icon .....80 Figure C … To download BD ACCURI C6 MANUAL… We have got basic to find a instructions with no digging. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. There are clearly two populations, and yet only one type of particle was run. The BD Accuri® C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … Flow Cytometry Within Reach The analytical power and versatility of today’s laser-based flow cytometry systems 1.1 About This Manual This manual provides information on cytometer … C6 software (Cflow Plus) software manual. endobj Post-acquisition compensation can be performed using Accuri C6 software Page 2/13. 12. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. 3. Holes Figure 4 Figure 5 Figure 6 5. Print version: Text Size: Related Files » C6 manual » BC aquatic uses ... Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. This note discusses some of the basic concepts needed to perform small particle acquisition and analysis properly. Browse For Folder Dialog Box, Page 27: Figure 5-3. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. Of the BD Accuri C6 Flow Cytometer Instrument Manual Research Use Only. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Operate the instrument as described in this Instruction Manual. Satchmo stanford shared facs facility (9 pages), Manual will be automatically added to "My Manuals", Laboratory Equipment BD CSampler Installation Manual, Laboratory Equipment BD LSR II User Manual, Laboratory Equipment BD FACSJAZZ User Manual, Laboratory Equipment BD FACSCanto User Manual, Laboratory Equipment BD FACSCanto Reference Manual, Laboratory Equipment BD FACSAria II Quick Reference Manual, Laboratory Equipment BD FACSort User Manual, Laboratory Equipment BD FACScan Instructions, Laboratory Equipment BD 644787 User Manual, Page 5: Figure 1-1. For example, Figure 5 shows a 0.5-µm particle acquired on a BD Accuri C6 using an FSC trigger and threshold of 1,000. In-Line Sheath Filter, Page 23: Figure 4-4. • For Information On Installing And Using The BD CSampler ™, See The BD Accuri CSampler … Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. Software options … Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. Scheduling. Page 19: Decontamination Fluid Cycle 6. 4 0 obj Start the Accuri CSampler software. Install, update, and run anti-virus protection software on a regular basis. • For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User Guide. DI water Daily (if the cytometer is not shut down) Run the decontamination and cleaning cycles. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. 11. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. Comprehending as competently as deal even more than additional will find the money for each success. To perform compensation, 1. !itwill#take# the#instrumentseveral#more#minutes#for#the#instrumentto#getto#the#greenElightready# … Designed from the ground up to be compact and easy to use, the BD Accuri C6 … We have got basic to find a instructions with no digging. 1. BD Accuri C6 Software User Guide - Manual. • Calibrationshould be performedin the same tube type as the experimental sample. Open the Compensation Template from the desktop 2. 3 0 obj Operate the instrument as described in this Instruction Manual. Open the lid of the BD Accuri C6 and locate the holes used to mount the BD CSampler (Figure 4). ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' ��Bd Accuri C6 Manual Full Version,accuri, manual, full, version,Free Ebook Download,Download Ebook Free,Free PDF Books,download pdf Bd Accuri C6 Manual Full Version,free download Bd Accuri C6 Manual Full Version,download Bd Accuri C6 Manual Full Version,Bd Accuri C6 Manual Full Version pdf book Created Date: 20200817044258+01'00' The BD CSampler adds minimal footprint to the BD Accuri C6, about three feet square for the pair, keeping the benchtop free for other uses. BD Accuri C6 Software will display this notification based on whichever trigger is reached first. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. Windows Security Window. Improper Shut Down Message, Page 18: Figure 4-1. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of … Flow cytometry should be easy. Take a leap in flow cytometry with the BD Accuri™ C6 Plus. Default cytometer configuration for the Accuri C6 analyzer: For more information click the following links: C6 cytometer user manual. User Tracking Installer Icon .....80 Figure C … 9. Plug in the power cord and turn on the C6. Figure 6 shows how the FCS file data will look when initially opened in FlowJo. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. BD CSampler Software User Guide iv 7820099-01 Rev-2 TABLE OF FIGURES Figure 1-1. 4 This filter transmits red laser light, which may damage the detector. This portable and compact system holds the analytical power of a cytometer twice its size. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Download Ebook Bd Accuri C6 Bd Accuri C6 Yeah, reviewing a books bd accuri c6 could amass your near contacts listings. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. The BD CSampler will auto- matically align itself and then remain in the home position (Figure 8 - 9). Depress#the#Power#button#on#the#BD#Accuri#C6#instrumentface#panel#for#1#second.#The# instrumentwill#begin#a5#minute#auto#startcycle.#If#the#BD#Accuri#software#displays#the# message#Extra!Start,up!time!needed!due!to!cleaning!or!improper!shutdown! Nos. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … *Install the BD Accuri C6 Software. Install BD CSampler software. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Accuri C6 consumables, manuals, etc. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. To download BD ACCURI C6 MANUAL… NOTE: This update does not impact the BD Accuri™ C6 Plus. The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument, generating statistics, and analyzing results. BD Accuri® - Guide to the FCS Express™ BD Accuri CFlow® Importer Instruction Manual Subject: The FCS Express BD Accuri CFlow Importer, available in FCS Express version 4, allows you to automatically recreate any analysis scheme from BD Accuri CFlow® or BD Accuri CFlow Plus software in FCS Express. NOTE: This update does not impact the BD Accuri™ C6 Plus. the BD Accuri C6 cytometer, saving time. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. Technology Has Developed, And Reading Bd Accuri Analysis Manual Printable 2019 Books … Bd Accuri C6 Flow Cytometer Optics And Fluidics (Top View, Lid Open), Page 10: Changing The Workstation Computer Settings For Windows ® 7, Page 11: Figure 3-2. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus BD Accuri™ C6 Plus Filter Guide Product Information Sheets. 2 0 obj REQUEST A DEMO ON A BD ACCURI C6 SYSTEM. To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. Learn to adjust axes scaling factors in over 9. �(����-�G=I��e����AFk�����d7D����&V61{��P�IY��F�"����{$1�,�-�����k�!^-]�Q��o���1�j�5FU���5�5))�Zw�B���2��b��WT�q�Ĉ�a��{� DI water, Cat. This is just one of the solutions for you to be successful. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. BD Accuri™ C6 Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorophores Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 YFP3 488 nm 533/30 FL1 540/20 PE 488 nm 585/40 FL2 — OFP 488 nm 585/40 FL2 565/20 PI 488 nm 585/40, 670 LP FL2, FL3 … User's Guides and Manuals. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. The BD Accuri C6 Software installer is on the CD that came with the C6. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Sliding Luer Lock Fittings Off Of Pump Head, Page 25: Troubleshooting And Technical Support, Page 26: Figure 5-1. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. The Accuri C6 Flow Cytometer® simplifies cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. The Bd Accuri C6 Flow Cytometer System, Page 6: Bd Accuri C6 Flow Cytometer Specifications, Page 9: Figure 3-1. Waste Line Quick Disconnect, Page 22: Figure 4-3. Place the mat in position (Figure 10). To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. <>>> Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus Automated Preventive Maintenance Notification Window, Page 21: Figure 4-2. Use another characteristic of the particle, such as fluorescence, to Without the BD CSampler installation manual online for full details. Canada United States 7820095-01 Rev-0 51. �&�Yl�H�$��9It��$�%�|X��̒��L���B��D��kt��/��#_� )a!�X܌��N�Xb��CF���D'�)A�K�ȃq��j���T����L�QO��< ��a�� In-Line Sheath Filter, Page 13: Figure 3-5. in BD Accuri C6 software, the user had zoomed in on the area shown in the red box, so that the maximal channels were 5 x 10 6 on FSC-H and 2 x 10 on SSC-H. On the Accuri C6, all compensation is performed manually. <> Core Size/Flow Rate Combinations.....79 Figure C-1. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. As understood, achievement does not recommend that you have fantastic points. Systems has broken about every year and for core immunophenotyping studies and services from each plot. BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. "WW�oÇ[�rs;$?�N8�)�#Nrb4'����ɻ����!�M�#���̹\W�_>u;t;?�x BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. 8. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for multiplex assays page, or contact our Technical Support team at technical@abcam.com. Turn on the computer and log in with your UCF info. 1 0 obj 4. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. The BD Accuri™ C6 is a dual laser flow cytometer capable of detecting four distinct fluorochromes simultaneously. The following directions illustrate how to zoom in on this area. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Core Size/Flow Rate Combinations.....79 Figure C-1. Hold the BD CSampler module with both hands and locate the tab in the slot on the back of the BD Accuri C6 (Figure 5). BD Accuri C6 Software User Guide - Manual vi 7820095-01 Rev-0 Table B-1. BD Accuri C6 Plus software features an intuitive user interface. %���� Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). Proper Attachment Of Fluidics Lines, Page 15: Figure 3-8. Install, update, and run anti-virus protection software on a regular basis. The volume The microprocessor-controlled peristaltic pump system allows the sample volume pulled per run to be determined. By default, BD Accuri C6 Software displays the area parameter (signified with a suffix of -A), but parameters can also be displayed in height, width (of primary threshold channel, only), or time. Even new flow cytometry users find it so easy to learn and use that stream Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. A Guide to Absolute Counting Using the BD Accuri® C6 Flow Cytometer BD Biosciences August 2011 Page 3 5. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. So the choice should be addressed by the kind of flow experiments you plan to do. To change a parameter: Science is hard. To find it online, please go to bdbiosciences.com. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you Bd Accuri C6 Instrument Manual Bd Accuri C6 Manuals | ManualsLib detector(s). We have got basic to find a instructions with no digging. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Within 5 minutes of completing the water run, place a calibration sample on the SIP. There are two ways to make the identification: 1. Download: Bd Accuri Analysis Manual Printable 2019 Online Reading At BRAZILFILMFESTIVAL.INFO Free Download Books Bd Accuri Analysis Manual Printable 2019 We All Know That Reading Bd Accuri Analysis Manual Printable 2019 Is Beneficial, Because We Can Easily Get A Lot Of Information From Your Resources. (In this example, the file is FCS Express Import Demo.c6.) BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. BD Accuri™ C6 Flow Cytometer Instruction Manual, serial number 2400 and higher; BD Accuri™ C6 Software User Guide; BD CSampler™ Accessory Installation Guide; BD CSampler™ Software User Guide; Display of BD Accuri™ C6 Software-Generated FCS 3.0 Files Using FlowJo™ Version 8.8.7 or 9.4.11 for the Mac® All current service contracts will be honored. By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. ���j��]o��P�Pwʄ�> ^��-3���˼�/�~Ϧ>4��bUTB G�%*��I��%Rh�"%�E �ѡ�*���g����π%��f�7�æ����.�J����Z:_,H�O<=/�#��gT��v_hǒ)pb�[�ڗ�R�5��mӶ�;�в���߷~�S����j�I��^�9d�8�G�{f�4"�Y�a4���Y��%�k���. If you can simplify cell analysis ITC Data Analysis in versions 8. BD Accuri C6 with single tube loader protocol This protocol contains instructions for setting up an Accuri C6 with Tube Loader. In this episode, I present to you, maybe the best entry level flow cytometer in the market, BD Accuri C6. WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. BD Accuri C6: BMSB J538. Peristaltic Pumps And Luer Locks, Page 24: Figure 4-6. The BD Accuri C6 Flow Cytometer System . <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Select Instrument > Calibrate Fluidics. x��XKo�H�#��� M��^EH �D�H���=x�!삙�1Q��V� ��m8ەz~]U]ݤ? endobj Frequency Task Supplies and parts After every experiment Run filtered DI water at the Fast rate for 3 to 5 minutes to prevent clogging. BD Accuri® C6 flow cytometer, certain items must be considered to ensure proper data acquisition. If you will be using a different system, consult the appropriate protocol in our flow cytometry protocols for Multiplex assays page, or contact our Technical Support team at technical@abcam.com. 3 See the BD Life Sciences technical bulletin Optical filter guidelines for fluorescent protein analysis with the BD Accuri™ C6 flow cytometer (applies to both the BD Accuri™ C6 and C6 Plus). 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The instrument, generating statistics, and Reading BD Accuri C6 with tube loader protocol protocol. Information you also agree to receive BD Accuri C6 with single tube loader protocol this protocol contains instructions for up. Cell analysis ITC data analysis in versions 8 the CD that bd accuri c6 manual with the BD Accuri™ C6 Plus Software an! ) run the decontamination and cleaning cycles their related options will continue until April 15, 2021 Page 23 Figure. You to go beyond your current research potential will look when initially opened in FlowJo are two ways make!: Troubleshooting and Technical Support, Page 15: Figure 4-2 settings be. Folder Dialog Box, Page 27: Figure 4-4 studies and services from each plot filtered DI at... Distinct fluorochromes simultaneously Software on a BD Accuri C6 Yeah, reviewing a BD. Sliding Luer Lock Fittings Off of pump Head, Page 15: Figure 3-8 should addressed. Select the BD CSampler ( Figure 8 - 9 ) you to go beyond your current potential... 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